Canola Phytase: Isolation and Characterization |
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| Authors: | H. KIM N.A.M. ESKIN |
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| Affiliation: | Authors Kim and Eskin are with the Dept. of Foods &Nutrition, Faculty of Human Ecology, Univ. of Manitoba, Winnipeg, Manitoba, Canada;. |
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| Abstract: | Two phytase isoenzymes were isolated from 8-day germinated canola cv Regent. Gel filtration chromatography of an ammonium sulfate fractionated extract on Sephadex G-100 produced one peak with phytase activity. The phytase fraction was separated into two isoenzymes by DEAE-cellulose chromatography. The optimum pH was 4.5–5.0 and 5.0 for the phytase isoenzymes 1 and 2, respectively. Both isoenzymes exhibited maximum activity at 50°C. Km values at pH 5.0 were 0.36 and 0.25 mM for phytase 1 and 2 isoenzymes, respectively, while molecular weight determination showed both fraction were identical with a molecular weight of 70,100 ± 4,000 daltons. |
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