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| HPLC-ELSD法测定苦杏仁中苦杏仁苷的含量 | |
| 引用本文： | 李海龙,王静,张英春,金鸿道,刘术明,郝东海.HPLC-ELSD法测定苦杏仁中苦杏仁苷的含量[J].食品与发酵工业,2007,33(1):109-112. |
| 作者姓名： | 李海龙 王静 张英春 金鸿道 刘术明 郝东海 |
| 作者单位： | 1. 黑龙江贝因美乳业有限公司,黑龙江安达,150036 2. 中国农业科学院农业质量标准与检测技术研究所,北京,100081 3. 哈尔滨工业大学食品科学与遗传工程研究院,黑龙江哈尔滨,150086 |
| 摘 要： | 建立了苦杏仁中苦杏仁苷的高效液相色谱-蒸发光散射检测器检测方法。采用ZORBAX Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm),流动相为V(甲醇)∶V(水)=40∶60,流速1.0mL/min,柱温40℃;蒸发光散射检测器漂移管温度60℃,载气(空气)压力0.2MPa。在上述条件下测得苦杏仁苷在0.28～5.5μg范围内的线性关系良好(r=0.9975),检出底限达58ng/L,平均加标回收率为96.2%。该方法简便快捷,重现性和准确度良好,可用于实际工作中样品的测定。 |
| 关 键 词： | 高效液相色谱 蒸发光散射检测器 苦杏仁 苦杏仁苷 |
| 修稿时间： | 09 6 2006 12:00AM |
Determination of Amygdalin in Semen Bitter Almond by High Performance Liquid Chromatographic Method with Evaporative Light Scattering Detector |
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| Li Hailong,Wang Jing,Zhang Yingchun,Jin Hongdao,Liu Shuning,Hao Haidong.Determination of Amygdalin in Semen Bitter Almond by High Performance Liquid Chromatographic Method with Evaporative Light Scattering Detector[J].Food and Fermentation Industries,2007,33(1):109-112. | |
| Authors: | Li Hailong Wang Jing Zhang Yingchun Jin Hongdao Liu Shuning Hao Haidong |
| Affiliation: | 1.Heilongjiang Beingmate Dairy Co., Ltd, Anda 150036, China;2.Institute of Quality Standards and Testing Technology for Agri- Products,Chinese Academy of Agricultural Science, Beijing 100081,China;3.Harbin Institute of Technology Food Science Academe, Harbin 150090, China |
| Abstract: | A high performance liquid chromatographic method with evaporative light scattering detector(ELSD)was established for determination of Amygdalin in Bitter Almond.Amygdalin was successfully separated by a XDB-C18 column(4.6x150mm,5um)at 40℃,as mobile phase composition with a binary solvent mixture of methanol-water(40∶60,volume ratio).The flow rate was 1.0mL/min.The ELSD drift tube temperature was 60oC, carrier air pressure was 0.2MPa.In this condition the linear range was 0.28～5.5μg(r=0.997 5).The detection limit was 58ng and the average recovery was 96.2%(RSD=2.6%,n=4).This method was accurate,rapid,simple,and reproducible。It is suitable for the sample detection in practice. |
| Keywords: | high performance liquid chromatography evaporative light scattering detection semen armeniacae amature amygdalin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |