| ELISA法测定组胺的不确定度评定 |
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| 引用本文： | 邹泽红,何颖,陈惠芳,黄于艺,刘雪婷.ELISA法测定组胺的不确定度评定[J].现代仪器,2013(2):50-51,56. |
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| 作者姓名： | 邹泽红 何颖 陈惠芳 黄于艺 刘雪婷 |
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| 作者单位： | 广州医学院第二附属医院/呼吸疾病国家重点实验室变态反应研究室/广州市过敏反应与临床免疫重点实验室 |
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| 基金项目： | 国家科技重大专项重点课题(项目编号:2009ZX08011-004B);广东省医学科研基金课题(项目编号:A2008284);广州市教育系统科研创新学术团队(项目编号:B94118) |
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| 摘 要： | 根据JJF1059-1999《测量不确定度评定与表示》规范对ELISA法测定组胺的不确定度进行评价分析,分别计算各分量的不确定度,进而计算合成不确定度。取k=2(置信概率95%),当样品rA17的组胺浓度为11.47μg/mL时,移取溶剂相对不确定度为0.015μg/mL,重复性试验相对不确定度为0.0395μg/mL,曲线拟合的相对不确定度0.021μg/mL,合成相对标准不确定度为0.047μg/mL,其扩展不确定度为1.08μg/mL,建立适合ELISA法测定组胺浓度的不确定度分析。
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| 关 键 词： | ELISA法 不确定度 组胺 |
Evaluation of uncertainty for determination of histamine by ELISA |
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| Zou Zehong He Ying Chen Huifang Huang Yuyi Liu Xueting.Evaluation of uncertainty for determination of histamine by ELISA[J].Modern Instruments,2013(2):50-51,56. |
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| Authors: | Zou Zehong He Ying Chen Huifang Huang Yuyi Liu Xueting |
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| Affiliation: | Zou Zehong He Ying Chen Huifang Huang Yuyi Liu Xueting(Guangzhou Municipal Key Laboratory of Allergy & Clinical Immunology,Allergy Research Branch of the State Key Laboratory of Respiratory Disease,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou 510260) |
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| Abstract: | The uncertainty for determination of histamine by ELISA was studied based on "Evaluation and Expression of Uncertainty in Measurement"(JJF1059-1999).The combined uncertainty was obtained by combining all standard uncertainty,and the expanded uncertainty was calculated by using a coverage of factor k=2,giving a level of confidence of approximately 95%,then to establish the evaluation of uncertainty which is suitable to measure the concentrations of histamine by ELISA.The uncertainty for determination of transfering solution and repeatability test and curve fitting,respectively,is 0.015 μg/mL and 0.0395 μg/mL and 0.021 μg/mL.Synthesis of relative standard uncertainty is 0.047 μg/mL. |
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| Keywords: | ELISA Uncertainty Histamine |
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