Quantitative determination of cyclopropenoid fatty acids in cottonseed meal |
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Authors: | R. S. Levi H. G. Reilich H. J. O’Neill Alva F. Cucullu E. L. Skau |
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Affiliation: | 1. IIT Research Institute, Chicago, Illinois 2. Southern Regional Research Laboratory, New Orleans, Louisiana
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Abstract: | A rapid analytical procedure for determining the residual cyclopropenoid fatty acids (CPA) in cottonseed meal has been developed. The procedure involves room-temperature extraction of crude CPA-containing lipids with a hexane-water-acetone azeotrope solvent, followed by a benzenemethanol wash. The crude lipids are then converted to methyl esters by methanolysis with sodium methoxide. Extraction with petroleum ether, followed by washing with aqueous acetone, results in a substance which is free from interfering materials. The purified methyl esters are then analyzed for CPA by a spectrophotometric modification of the Halphen reaction. |
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