The effect of the EAAEAE insert on the property of human metallothionein-3 |
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| Authors: | Zheng Qi; Yang Wan-Ming; Yu Wen-Hao; Cai Bin; Teng Xin-Chen; Xie Yi; Sun Hong-Zhe; Zhang Ming-Jie; Huang Zhong-Xian |
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| Affiliation: | 1Chemical Biology Laboratory, Department of Chemistry and
3Institute of Genetics, Life School, Fudan University, Shanghai 200433,
2Department of Chemistry, Kunming University of Science and Technology, Kunming,
4Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong and
5Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PR China |
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| Abstract: | MT3 shows apparently different properties and function fromMT1 even though they have 70% sequence homology. Possibly thetwo inserts, Thr5 and a negatively charged hexapeptide at position-55in MT3, play important roles. A series of MT3 variants aroundthe EAAEAE hexapeptide have been prepared by site-directed mutagenesisand their properties and reactivity towards pH, EDTA and DTNBhave been studied. Our detailed studies revealed that the EAAEAEinsert is essential to the property of MT3. It is the hexapeptideinsert, to some extent, making the MT3 |
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