除铁锰菌的分离及其氧化性能的实验

目的检测从成熟锰砂上分离得到的细菌的形态结构和其对铁锰氧化过程各因素的变化，深入了解微生物除铁锰的过程及特性，为实际工程中微生物除铁锰提供参考依据．方法采用PYCM培养基和革兰氏染色法进行细菌的分离和鉴别，将分离出来的细菌与其混合菌在条件相同的情况下做对比实验，定期取样观察各细菌氧化铁锰时各种因素的变化．结果从成熟锰砂上分离出了4种细菌，分别为白色、黄色、橙红色和粉色；这4种菌对铁的氧化过程都呈现出相似的周期性，细菌在氧化铁锰时所引起溶液pH的变化趋势与细菌在氧化锰过程中锰去除率变化曲线十分相似．结论混合菌的氧化作用比单一菌的氧化作用强．细菌数量的变化，以及与铁锰相互作用的结果都影响着溶液的pH，通过pH的升高或降低，可以了解到细菌对铁锰作用的强弱以及细菌数量的增加与减少．细菌对铁锰的氧化作用的强弱，不仅决定于细菌数量的多少．而且也决定于细菌的种类以及溶液的pH．

Separation and Oxidizability Property of Iron Removing and Manganese Removing Bacteria

The purpose of the study is to know the course of the biological removal of iron and manganese.Four strains of bacteria,which show similar periodicity in their oxidation on iron,are obtained on the surface of mature manganese sand.Their structures were examined and the effects of time on the removal of iron and manganese were also checked so as to select the most effective iron and manganese removing bacteria.The oxidation of mixed bacteria is stronger than that of unitary bacteria.The change of bacteria quantity as well as the interaction between iron and manganese affects pH of the solution.The change of the pH helps us to know the effect of the germs on iron and manganese as well as the increase and decrease of the quantity of the bacteria.The bacteria's oxidation of iron and manganese is not only subject to bacteria quantity,but to pH of the solution and the strain of the bacteria as well.