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Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants
Authors:Hung-Der Jang  Ku-Shang Chang  Yung-Sheng Huang  Chuan-Liang Hsu  Sheng-Hsien Lee  Min-Sheng Su
Affiliation:1. Department of Food Science, Yuanpei University, 360 Yuanpei Street, Hsinchu 300, Taiwan;2. Institute of Biotechnology, Yuanpei University, Hsinchu 300, Taiwan
Abstract:The principal antioxidant components and content of cinnamon (Cinnamomum cassia), turmeric (Curcuma longa) and golden thread (Coptidis rhizoma) extracts were determined using high performance liquid chromatography (HPLC) with UV detection. In general, C. cassia, C. longa and C. rhizoma extracts from domestic Taiwan were rich in cinnamaldehyde, curcumin, and berberin, respectively. The contents of cinnamaldehyde, curcumin, and berberin in the acetone extracts were 1911, 2029, and 840 mg l−1, respectively. The Folin–Ciocalteu method was used to measure the total phenolic concentrations of extracts, which had the content of 9.6 (C. cassia), 2.6 (C. longa), and 4.3 (C. rhizoma) mM l−1. In addition, DPPH radical-scavenging, ferric-reducing antioxidant power (FRAP), and ferric thiocyanate (FTC) assays were employed to measure antioxidant activities. The C. cassia fresh extracts had higher antioxidant activities which were 84–90% (DPPH), 17–33 μmol l−1 (FRAP), and 53–82% (FTC). The activities of C. longa fresh extracts were 22–44% (DPPH), 7–11 μmol l−1 (FRAP), and 53–81% (FTC) while C. rhizoma were 53–64% (DPPH), 18–26 μmol l−1 (FRAP), and 59–82% (FTC).
Keywords:Cinnamaldehyde  Berberin  Curcumin  HPLC  Total phenols  Antioxidant activity
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