Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures |
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Authors: | Nicola E Durling Owen J Catchpole John B Grey Rosemary F Webby Kevin A Mitchell L Yeap Foo Nigel B Perry |
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Affiliation: | 1. Industrial Research Ltd., Gracefield Research Centre, P.O. Box 31-310, Lower Hutt, New Zealand;2. Plant Extracts Research Unit, New Zealand Institute for Crop and Food Research Ltd., Department of Chemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand |
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Abstract: | Effects of particle size, temperature, contact time, solvent-to-sage ratio and the ethanol–water ratio on the extraction of the active compounds rosmarinic acid, carnosic compounds and essential oil from dried sage (Salvia officinalis) were studied. Optimal extraction conditions giving highest yield of all three active compounds were particle diameter 1 mm, extraction temperature 40 °C, solvent-to-sage ratio of 6:1 and 55–75 wt% ethanol for up to 3 h. This gave an extract equivalent to 14.9% of dry sage, containing 6.9% rosmarinic acid (55% recovery), 10.6% carnosic compounds (75% recovery) and 7.3% essential oil (42% recovery). Scale up of the process by a factor of 100 demonstrated that the optimised laboratory scale process can be carried out without any loss of efficiency at an industrial scale. |
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Keywords: | Sage Salvia officinalis Phenolics Rosmarinic acid Solvent extraction Essential oil |
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