CdTe/CdS量子点对木犀草素与人血清白蛋白结合力的影响

用还原型谷胱甘肽(GSH)作硫源和稳定剂在水相中合成了生物相容性好、量子产率高的核壳型CdTe/CdS半导体量子点;同时模拟人体内生理环境,研究了CdTe/CdS量子点(QDs)对木犀草素与人血清白蛋白(HSA)相互作用的影响。结果表明,CdTe/CdS QDs可有规律地猝灭HSA荧光;在CdTe/CdS QDs存在条件下,木犀草素可使HSA荧光最大发射波长从341 nm红移到347 nm,红移程度明显大于无QDs存在时,同时木犀草素与HSA的表观结合常数和结合位点数均有增加,即CdTe/CdS QDs使木犀草素与HSA的结合力增强。

The Preparation and Application of CdTe/CdS Core-shell Quantum Dots

Biocompatible and high luminescence CdTe/CdS core -shell quantum dots （ QDs ） were synthesized in aqueous solution using glutathione （ GSH） as sulfur source and stabilizer.Further, luteolin was studied for the affinities for human serum albumin （ HSA） in the presence and absence of CdTe/CdS QDs, respectively.Results revealed that the fluorescence intensities of HSA decreased remarkably with increasing concentration of QDs.Luteolin resulted in obvious red-shift of the maximum emission of HSA from 341 nm to 347 nm in the presence of QDs and the extent of red -shift induced by luteolin was much bigger than that in the absence of QDs.The binding constants and the number of binding sites per HSA molecule （ n） both increased.Thus, the binding affinities of luteolin for HSA increased in the presence of CdTe/CdS QDs.