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Suppressive effect of hot water extract of wasabi (Wasabia japonica Matsum.) leaves on the differentiation of 3T3-L1 preadipocytes
Authors:Tetsuro Ogawa   Hiromasa Tabata   Takuya Katsube   Yukari Ohta   Yukikazu Yamasaki   Masayuki Yamasaki  Kuninori Shiwaku
Affiliation:aShimane Institute for Industrial Technology, 1 Hokuryo-cho, Matsue City, Shimane 690-0816, Japan;bApplied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, 1677-1, Yoshida, Yamaguchi City, Yamaguchi 753-8515, Japan;cDepartment of Environmental and Preventive Medicine, Shimane University School of Medicine, 89-1 Enya-cho, Izumo City, Shimane 693-8501, Japan
Abstract:This study investigated the effect of hot water extract of wasabi (Wasabia japonica Matsum.) leaves (WLE), without its specific pungent constituents, such as allyl isothiocyanate, on the differentiation of 3T3-L1 preadipocytes. WLE suppressed the increase in glycerol-3-phosphate dehydrogenase (GPDH) activity and triglyceride (TG) accumulation, markers of adipogenesis, in a dose-dependent manner. Quantitative real time RT-PCR results showed that WLE significantly reduced the mRNA expression levels of peroxisome proliferator-activated receptor (PPAR) γ and CCAAT/enhancer-binding protein (C/EBP) α, both key adipogenic transcription factors, as subsequently were the mRNA expression levels of their target genes, such as adipocyte fatty acid binding protein 2 (aP2). Western blot analysis results showed that the protein expression levels of both PPARγ and C/EBPα were also inhibited by WLE. Thus, WLE suppressed the differentiation of 3T3-L1 preadipocytes, and the suppressive effect was mediated, in part, through the altered regulation of PPARγ, C/EBPα, and other specific genes, such as aP2. These results suggest that WLE may prevent obesity and insulin resistance by inhibiting the differentiation of preadipocytes.
Keywords:Wasabi   3T3-L1 cell   Adipocyte   Differentiation   PPARγ     C/EBPα  
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