Improved Reaction Conditions for Preparation of β-Cyclodextrin (β-CD) from Cassava (Manihot esculenta Crantz) Starch

Experimental conditions for bioconversion of starch to get higher yield of ß-cyclodextrin using cyclodextrin glycosyl transferase (CGT-ase) (EC 2.4.1.19) were worked out. A 5% initial starch concentration gave ß-CD yield of 20-28% that could be separated as ß-CD bromobenzene complex from the reaction mixture. Addition of bromobenzene during the second phase of the reaction, i.e. CD formation phase, was found beneficial.