Net lipid transfer between lipoproteins in fish-eye disease plasma supplemented with normal high density lipoproteins |
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Authors: | Leif Holmquist Lars A Carlson |
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Affiliation: | (1) King Gustaf V Research Institute, Karolinska Institute and Karalinska Hospital, Box 60004, S-104 01 Stockholm, Sweden;(2) Department of Internal Medicine, Karolinska Institute and Karolinska Hospital, Stockholm, Sweden |
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Abstract: | Native fish-eye disease plasma, which is deficient of both high density lipoproteins (HDL) and lecithin-cholesterol acyltransferase
activity (α-LCAT), processing the free cholesterol of these lipoproteins, has been supplemented with normal isolated HDL2 or HDL3 and incubated in vitro at 37 C. After incubation for 0,7.5 and 24 hr the very low density (VLDL) and low density (LDL) lipoproteins
as well as HDL were isolated, and their contents of triglycerides, phospholipids and free, esterified and total cholesterol
were quantified. The resulting net mass transfer of the different lipids revealed a functioning transfer of cholesteryl esters
and all other analyzed lipids between the lipoproteins, although no de novo esterification of the HDL cholesterol by LCAT
in this plasma occurred. In accordance with previous findings there was a functioning esterification process of the free cholesterol
of the combined VLDL and LDL of fish-eye disease plasma. The present results make it reasonable to conclude that the lack
of HDL cholesterol esterification in this disease is not a result of a deficiency of cholesteryl ester transfer or lipid transfer
activities. |
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