Antioxidant, antihypertensive and antimicrobial properties of ovine milk caseinate hydrolyzed with a microbial protease |
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Authors: | Corrêa Ana Paula F Daroit Daniel J Coelho Julise Meira Stela M M Lopes Fernanda C Segalin Jéferson Risso Patrícia H Brandelli Adriano |
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Affiliation: | Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. |
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Abstract: | BACKGROUND: Bioactive peptides might be released from precursor proteins through enzymatic hydrolysis. These molecules could be potentially employed in health and food products. In this investigation, ovine milk caseinate hydrolysates obtained with a novel microbial protease derived from Bacillus sp. P7 were evaluated for antioxidant, antimicrobial, and angiotensin I‐converting enzyme (ACE)‐inhibitory activities. RESULTS: Antioxidant activity measured by the 2,2′‐azino‐bis‐(3‐ethylbenzothiazoline)‐6‐sulfonic acid method increased with hydrolysis time up to 2 h, remaining stable for up to 4 h. Hydrolysates showed low 2,2‐diphenyl‐1‐picrylhydrazyl radical‐scavenging abilities, with higher activity (31%) reached after 1 h of hydrolysis. Fe2+‐chelating ability was maximum for 0.5 h hydrolysates (83.3%), decreasing thereafter; and the higher reducing power was observed after 1 h of hydrolysis. ACE‐inhibitory activity was observed to increase up to 2 h of hydrolysis (94% of inhibition), declining afterwards. 3 h hydrolysates were shown to inhibit the growth of Bacillus cereus, Corynebacterium fimi, Aspergillus fumigatus, and Penicillium expansum. CONCLUSION: Ovine caseinate hydrolyzed with Bacillus sp. P7 protease presented antioxidant, antihypertensive, and antimicrobial activities. Hydrolysis time was observed to affect the evaluated bioactivities. Such hydrolysates might have potential applications in the food industry. Copyright © 2011 Society of Chemical Industry |
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Keywords: | protein hydrolysates bioactive peptides antioxidant activity antihypertensive activity antimicrobial activity |
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