<Emphasis Type="Italic">Penicillium</Emphasis> sp. ZD-Z1 chitosanase immobilized on DEAE cellulose by cross-linking reaction |
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Authors: | Email author" target="_blank">Lian-Ying?ZhengEmail author Yu-Liang?Xiao |
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Affiliation: | (1) Department of Chemical and Biochemical Engineering, Zhejiang University, 310027 Hangzhou, P.R. China |
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Abstract: | Chitosanase obtained fromPenicillium sp.ZD-Z1 was immobilized on DEAE cellulose with glutaraldehyde by cross-linking reaction. The optimal conditions of immobilization
were as follows: 0.1 g DEAE cellulose was treated with 5 ml 5% glutaraldehyde solution; then 2.3 mg chitosanase was immobilized
on the carrier. The optimal temperature and pH was 60 °C and 4.0, and the K
m
value was 18.87 g/L. Under optimal conditions, the activity of immobilized enzyme is 1.5 U/g, and the recovery of enzyme
activity is 81.3%. After immobilization, the optimal temperature and K
m
value increased (from 50 °C to 60 °C, from 2.49 g/L to 18.87 g/L), whereas the optimal pH was reduced (from 5.0 to 4.0).
The enzyme activity loss was less than 20% after 10 times batch reaction; the immobilized enzyme showed good operation stability. |
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Keywords: | DEAE Cellulose Immobilization Chitosanase Chitosan |
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