| 兔血中5β-H-17-OH雄甾烷的气相色谱-质谱测定 |
| |
| 引用本文： | 骆传环,黄荣清,肖炳坤,梁乾德,梁晓东.兔血中5β-H-17-OH雄甾烷的气相色谱-质谱测定[J].质谱学报,2005,26(1):46-46. |
| |
| 作者姓名： | 骆传环 黄荣清 肖炳坤 梁乾德 梁晓东 |
| |
| 作者单位： | 军事医学科学院放射医学研究所,北京,100850 |
| |
| 摘 要： | 建立了一种气相色谱 -质谱 (GC/MS)技术测定兔血中甲睾 (MT)的主要代谢物 5 β-H-1 7-OH雄甾烷(5β-MT)质量浓度的方法 ,为药物动力学等研究提供技术方法和数据。 6只兔口服 2 0 mg MT后按设定时间点取血 ,用甲醇提取血浆 1 m L,提取液用 β-葡萄糖醛酸苷酶于 5 5℃水解 3 h。去氢甲睾 (DHMT)为内标物进行定量计算。结果显示 :给药后每个血样中均含有 5 β-MT,计算每个时间点 6只兔的 5 β-MT平均质量浓度 ρ,发现在 3 h时达到峰值 2 .2 mg/L
|
| 关 键 词： | 5β-H-17-OH雄甾烷 气相色谱-质谱(GC/MS) 内标定量 质量浓度 兔血 |
| 文章编号： | 1004-2997(2005)01-46-04 |
| 收稿时间： | 2004-03-06 |
Determination of 5β-H-17-OH-Androstane in Rabbit Blood by Gas Chromatography-Mass Spectrometry |
| |
| LUO Chuan-huan,HUANG Rong-qing,XIAO Bing-kun,LIANG Qian-de,LIANG Xiao-dong.Determination of 5β-H-17-OH-Androstane in Rabbit Blood by Gas Chromatography-Mass Spectrometry[J].Journal of Chinese Mass Spectrometry Society,2005,26(1):46-46. |
| |
| Authors: | LUO Chuan-huan HUANG Rong-qing XIAO Bing-kun LIANG Qian-de LIANG Xiao-dong |
| |
| Abstract: | The determination of the essential metabolite 5β-H-17-OH-Androstane (5β-MT) from methyl testosterone (MT) in rabbit blood by gas chromatography-mass spectrometry was performed to supply method and data for pharmacokinetics study. 2mL blood taken from six rabbits ear was collected at setting time point before and after P.O. administration 20mg MT,1mL plasma sample was extracted with methanol, then the extractant was enzymed by β-glucuronidase. Quantitative analysis was performed with internal standard DHMT. The result showed that 5β-MT was presented in each blood sample, the maximal average mass concentration of six rabbit is 2.2mg/L at 3h. |
| |
| Keywords: | 5β-H-17-OH-androstane gas chromatography-mass spectrometry internal standard quanitation mass concentration rabbit blood |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《质谱学报》浏览原始摘要信息 |
|
点击此处可从《质谱学报》下载全文 |
|
