Effect of acylation stimulating protein on the triacylglycerol synthetic pathway of human adipose tissue |
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| Authors: | Zivart Yasruel Katherine Cianflone Allan D Sniderman Mark Rosenbloom Mark Walsh Miguel A Rodriguez |
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| Affiliation: | (1) The McGill Unit for the Prevention of Cardiovascular Disease, McGill University, Royal Victoria Hospital, H3A 1A1 Montreal, Quebec, Canada;(2) Biochemistry Department, University of Ottawa, 31 George Glinski, K1N 6N5 Ottawa, Ontario |
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| Abstract: | Acylation stimulating protein (ASP) is a 14 kDa plasma protein which causesin vitro triacylglycerol synthesis in human adipocytes and fibroblasts to increase substantially. ASP was found to stimulate human
adipose tissue microsomal glycerophosphate acyltransferase and diacylglycerol acyltransferase activities by 23% and 90%, respectively.
However, phosphatidate phosphohydrolase activity showed no increase in activity, nor did microsomal acyl-CoA synthetase activity.
Moreover, ASP did not decrease the apparent Km of diacylglycerol acyltransferase (DGAT), but rather increased its apparent Vmax suggesting direct interaction of ASP with DGAT. |
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