Cs corrected STEM EELS: Analysing beam sensitive carbon nanomaterials in cellular structures |
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| Authors: | Mhairi H Gass Alexandra E Porter James S Bendall Karin Muller Jeremy N Skepper Paul A Midgley Mark Welland |
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| Affiliation: | 1. SuperSTEM, Daresbury Lab, Keckwick Lane, Daresbury WA4 4AD, UK;2. Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK;3. The Nanoscience Centre, University of Cambridge, 11 J.J. Thompson Avenue, Cambridge CB3 OFF, UK;4. Multiimaging Centre, Department of PDN (Physiology, Development and Neuroscience), Anatomy Building, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK;5. Department of Materials Science and Metallurgy, University of Cambridge, Pembroke Street, Cambridge CB2 3QZ, UK |
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| Abstract: | Identification of individual single wall nanotubes (SWNTs) within a cellular structure can provide vital information towards understanding the potential mechanisms of uptake, their localisation and whether their structure is transformed within a cell. To be able to image an individual SWNT in such an environment a resolution is required that is not usually appropriate for biological sections. Standard transmission electron microscopy (TEM) techniques such as bright field imaging of these cellular structures result in very weak contrast. Traditionally, researchers have stained the cells with heavy metal stains to enhance the cellular structure, however this can lead to confusion when analysing the samples at high resolution. Subsequently, alternative methods have been investigated to allow high resolution imaging and spectroscopy to identify SWNTs within the cell; here we will concentrate on the sample preparation and experimental methods used to achieve such resolution. |
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| Keywords: | STEM Electron microscopy EELS Energy loss HAADF SWNT Nanotubes Toxicity Nanomedicine |
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