| SIRT3多酚激活剂的抗细胞氧化应激作用及机制 |
| |
| 引用本文： | 陈慧,彭雨欣,徐莹皓,赵冲.SIRT3多酚激活剂的抗细胞氧化应激作用及机制[J].中国食品学报,2022,22(1):12-20. |
| |
| 作者姓名： | 陈慧 彭雨欣 徐莹皓 赵冲 |
| |
| 作者单位： | 中国农业大学食品科学与营养工程学院 北京 100083 |
| |
| 基金项目： | 国家自然科学基金青年科学基金项目(31901691) |
| |
| 摘 要： | 建立Caco-2单层细胞与HaCaT细胞形成的迁移小室(trans-well)共培养体系,采用HaCaT细胞SIRT3-EGFP报告基因系统评价5种膳食多酚(白藜芦醇、山奈酚、安石榴苷、漆树黄酮、月见草素B)对SIRT3基因表达的影响,并进一步评价筛选的SIRT3多酚激活剂对UVB诱导的HaCaT细胞氧化应激的抑制作用...
|
| 关 键 词： | 多酚 SIRT3 细胞共培养 氧化应激 |
| 收稿时间： | 2021/1/18 0:00:00 |
Inhibitive Effects of SIRT3-augmenting Polyphenols on Cellular Oxidative Stress |
| |
| Chen Hui,Peng Yuxin,Xu Yinghao,Zhao Chong.Inhibitive Effects of SIRT3-augmenting Polyphenols on Cellular Oxidative Stress[J].Journal of Chinese Institute of Food Science and Technology,2022,22(1):12-20. |
| |
| Authors: | Chen Hui Peng Yuxin Xu Yinghao Zhao Chong |
| |
| Affiliation: | College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083 |
| |
| Abstract: | In this study,the trans-well co-culture system of Caco-2 monolayer cells and HaCaT cells was established,and the effects of resveratrol,kaempferol,punicalagin,fisetin and oenothein B on SIRT3 expression were evaluated by the SIRT3-EGFP reporter gene system in HaCaT cells,as well as the protective effect of the selected SIRT3-augmenting polyphenols on UVB-induced oxidative stress in HaCaT cells were further investigated.The result showed that resveratrol,kaempferol and oenothein B significantly enhanced the expression of exogenous and endogenous SIRT3 gene;among them,oenothein B significantly reduced the level of ROS in HaCaT cells;however,knockdown SIRT3 expression significantly reduced the inhibitory effect of oenothein B on UVB-induced oxidative stress.In conclusion,the inhibition effect of oenothein B on UVB-induced ROS generation depends on SIRT3 in the co-culture system of Caco-2 cells and HaCaT cells. |
| |
| Keywords: | polyphenols SIRT3 cells co-cluture ROS |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《中国食品学报》浏览原始摘要信息 |
|
点击此处可从《中国食品学报》下载全文 |
|
