Development of a multichannel fluorescence affinity sensor system |
| |
Authors: | Schuderer Akkoyun Brandenburg Bilitewski Wagner |
| |
Affiliation: | Fraunhofer-Institut fur Physikalische Messtechnik IPM, Freiburg, Germany. |
| |
Abstract: | A multichannel fluorometer is proposed for analysis of biochemical reactions. The sensor is based on the luminescence generation in the evanescent field of a totally reflected laser beam. For transduction, multiple reflection elements are used. Multichannel operation is realized, including the possibility of applying different solutions to each channel at the same time. First experimental results, obtained with fluorescein or Cy5 as labels in a model hybridization assay, demonstrate the applicability and allow the detection of 3-10 fmol injected fluorescently labeled oligonucleotide. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|