大孔树脂吸附纯化葡萄籽原花青素的研究

采用大孔树脂对葡萄籽原花青素进行吸附纯化,以吸附率和解吸率及纯化效果为指标,比较了7种大孔树脂对原花青素的吸附解吸性能。研究了上样量、洗脱剂浓度、上样流速、洗脱剂用量对原花青素纯化效果的影响。结果表明,AB-8大孔树脂对葡萄籽中原花青素的吸附效果最好,树脂体积为20 mL,上样量为80 mL,上样流速为40 mL/h,用40%的乙醇洗脱,洗脱剂用量为100 mL,洗脱流速为80 mL/h时的纯化效果最好,该条件下原花青素洗脱率为94.37%,所得溶质纯度可达89.63%。

Study on the purification to procyanidins which are extracted from grape seeds by macroporous adsorbent resin

It was studied to purify procyanidins which are extracted from grape seeds by macroporous adsorbent resin.The adsorption and desorption performance of procyanidins on 7 kinds of macroporous adsorbent resins were compared.The different factors influencing procynidins purity including solution volume,ethanol concentration,solution velocity,eluting dosage were investigated.The optimum technical conditions were obtained through orthogonal experiments.AB-8 macroporous resin was an excellent adsorbent resin,resin volume was 20 mL,solution volume was 80 mL,solution velocity was 40 mL/h,the flow rate was 80 mL/h of 40% ethanol solution by 100 mL.94.37% Procyanidins could be desorbed from AB-8 macroporous resin and the purity of procyanidins reached 89.63%.