Pilot batch production of specific-structured lipids by lipase-catalyzed interesterification: Preliminary study on incorporation and acyl migration |
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Authors: | X Xu S Balchen C -E Høy J Adler-Nissen |
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Affiliation: | (1) Department of Biochemistry and Nutrition, Technical University of Denmark, DK-2800 Lyngby, Denmark;(2) Department of Biotechnology, Technical University of Denmark, Building 221, DK-2800 Lyngby, Denmark |
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Abstract: | Effects of water content, reaction time, and their relationships in the production of two types of specific-structured lipids
(sn-MLM- and sn-LML-types: L-long chain fatty acids; M-medium chain fatty acids) by lipase-catalyzed interesterification in a solvent-free
system were studied. The biocatalyst used was Lipozyme IM (commercial immobilized lipase). The substrates used for sn-MLM-type were fish oil and capric acid, and medium chain triacylglycerols and sunflower free fatty acids for sn-LML-type. The observed incorporation with the time course agrees well with the Michaelis-Menten equation, while the acyl
migration is proportional to time within the range of 20 mol% acyl migration (MLM-type: M
f
=0.2225 T, R2=0.98; LML-type: M
f
=0.5618 T, R2=0.99). As water content (wt%, on the enzyme basis) increased from 3.0 to 11.6% for MLM-type and from 3.0 to 7.2% for LML-type
in the solvent-free systems, the incorporation rates in the first 5 h increased from 3.34 to 10.30%/h, and from 7.29 to 11.12%/h,
respectively. However, the acyl migration rates also increased from 0.22 to 1.12%/h and from 0.56 to 1.37%/h, respectively.
Different effects in the production of two totally position-opposed lipids can be observed. Presumably these are caused by
the different chain length of the fatty acids. The relationships between reaction time and water content are inverse and give
a quantitative prediction of incorporation and acyl migration in selected reaction conditions and vice versa. The acyl migration
can not be totally avoided in present systems, but can be reduced to a relatively low level. Acyl migration during the downstream
processing has also been observed and other factors influencing the acyl migration are briefly discussed. |
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Keywords: | Acyl migration incorporation lipase-catalyzed interesterification Lipozyme IM LML-type MLM-type pilot batch reactor specific-structured lipids |
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