絮状试验用白喉和破伤风类毒素标准的国际协作试验

按照国际协作试验计划,本试验用 Ramon 絮状试验方法标化了絮状试验用白喉类毒素和破伤风类毒素国际标准各一批,推荐品即 DIFT 和 TEFT。本试验室标定结果与由13个实验室参加的国际协作标定结果几乎相同。以 DIFT 和 TEFT 为标准,用 Levine-Wyman 的试管絮状试验法和火箭电泳测定了几批白喉类毒素和破伤风类毒素的 Lf,其结果可与 Ramon 方法测定结果相比,证明火箭电泳可用于定量测定类毒素(或毒素)的絮状单位。

Collaborative study on calibration of the proposed international reference materials of diphtheria and tetanus toxoid for flociation test

According to the program of international collaborative study on de-fining If-units for diphtheria and tetanus toxoids as international reference materi-als,we have calibrated the two proposed international reference toxoids (DIET andTEFT) by Ramon test.The results obtained from our laboratory were close to thoseobtsined from international collaborative assay by 13 laboratories.A number of diphtheria and tetanus toxoids were titrated both by Levine-Wy-man flocculation and rocket immunoelectrophoresis.These results were compared withthose obtained by Ramon test.It was demonstrated that the rocket immunoelectro-phoresis could be used as an alternative to the flocculation test.