乳酸发酵短杆菌中碳架流向赖氨酸的导向与分析

对乳酸发酵短杆菌生物合成赖氨酸的代谢途径及其代谢流量从理论上进行了分析，计算了乳糖发酵短杆菌合成赖氨酸的最大理论转化率。对代谢途径及其遗传调控进行了分析，通过NTG诱变育成了一株带有FP^S、AEG^r、Ma^r和Leu^-4种遗传标记的赖氨酸高产菌株，使赖氨酸的产量提高到54.6g/L，并分析了这些标记对代谢流量改变的影响。

Redirection and Analysis of the Carbon Flux to the Biosynthetic Pathway of Lysine in Brevibacterium lactofermentum

In this paper, the biosynthesis of lysine and the theoretical distribution of its metabolic flux were analyzed, and the theoretical yield of lysine in Brevibacterium lactofermentum was calculated to be 0.76. Based on the pathway analysis and the knowledge of its metabolic regulation, a lysine producer was obtained by mutagenesis with N methyl N nitronitrosoguanidine of strain 1230 with four genetic markers, FP s,AEC r,MA r and Leu -. After optimization of the fermentation conditions, the lysine production was increased to above 54.6 g/L, however, the emphasis of this work was put on the analysis of the effects of these genetic markers on the redirection of metabolic flux.