| 高效液相色谱法测定大蒜素片中DADS和DATS的含量 |
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| 引用本文： | 李天昊,李若琦,黄雪婷,陈梅兰. 高效液相色谱法测定大蒜素片中DADS和DATS的含量[J]. 分析仪器, 2021, 0(2): 116-119 |
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| 作者姓名： | 李天昊 李若琦 黄雪婷 陈梅兰 |
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| 作者单位： | 浙江树人大学生物与环境工程学院;江苏常州大学 |
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| 基金项目： | 国家市场监督管理总局技术保障专项项目(2020YJ014);浙江树人学院大学生创新创业训练计划。 |
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| 摘 要： | 建立并使用高效液相色谱法测定药用大蒜素片中二烯丙基二硫醚(Diallyl disulfide,DADS)以及二烯丙基三硫醚(Diallyl trisulfide,DATS)的含量。实验使用H ypersiL ODS C18柱进行色谱分离,流动相由甲醇/水/甲酸组成,比例为85∶15∶0.1。测定波长是225nm,流速为1.0 mg/L。实验结果表明,DADS在1.0~16.0mg/L浓度的范围内和峰面积有较好的线性关系,R^(2)=0.9991。DATS在1~40mg/L浓度的范围内和峰面积有较好的线性关系,R^(2)=0.9994。DADS的回收率在91.20%~98.80%,DATS的回收率在94.74%~106.39%。
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| 关 键 词： | 大蒜素 二烯丙基二硫醚 二烯丙基三硫醚 高效液相色谱法(HPLC) |
Determination of DADS and DATS in highly concentrated allicin tablets by HPLC |
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| Li Tianhao,Li Ruoqi,Huang Xueting,Chen Meilan. Determination of DADS and DATS in highly concentrated allicin tablets by HPLC[J]. Analytical Instrumentation, 2021, 0(2): 116-119 |
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| Authors: | Li Tianhao Li Ruoqi Huang Xueting Chen Meilan |
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| Affiliation: | (College of Biological and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China;College of Petrochemical,Jiangsu Changzhou University,Changzhou 213164,Jiangsu,China) |
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| Abstract: | The chromatographic separation was performed on a Hypersil ODS C18 column,with methanol,water and formic acid solution(v∶v∶v,85∶15∶0.1)as the mobile phase.The detection wavelength was 225 nm,and the flow rate was 1.0 mL/min.The test results showed that DADS had a good linear relationship with peak area in the range of 1.0—16.0mg/L,R2=0.9991;DATS had a good Linear relationship with peak area in the range of 1—40mg/L,R2=0.9994.The recoveries of DADS were 91.20%—98.80%,and the recoveries of DATS was 94.74%—106.39%. |
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| Keywords: | Garlic Diallyl disulfide(DADS) Diallyl trisulfide(DATS) HPLC |
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