Assessment of cell viability in a three-dimensional enzymatically cross-linked collagen scaffold |
| |
Authors: | Y Garcia R Collighan M Griffin A Pandit |
| |
Affiliation: | (1) National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland;(2) School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK;(3) Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Ireland |
| |
Abstract: | Microbial transglutaminase (mTGase) is an enzyme that introduces a covalent bond between peptide bound glutamine and lysine
residues. Proteins cross-linked in this manner are often more resistant to proteolytic degradation and show increased tensile
strength. This study evaluates the effects of mTGase mediated cross-linking of collagen on the cellular morphology, behaviour
and viability of murine 3T3 fibroblasts following their seeding into collagen scaffolds. Additionally, cell mediated scaffold
contraction, porosity and level of cross-linking of the scaffold has been analysed using image analysis software, scanning
electron microscopy (SEM), colorimetric assays, and Fourier transform infrared spectroscopy (FTIR). We demonstrate that the
biocompatibility and cellular morphology, when comparing cultures of fibroblasts integrated in mTGase cross-linked collagen
scaffolds with the native collagen counterparts, remained unaffected. It has been also elicited that the structural characteristics
of collagen have been preserved while introducing enzymatically resistant covalent bonds.
This work was performed in the National Centre for Biomedical Engineering Science, Orbsen Building, National University of
Ireland, Galway, Ireland. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|