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Core-Shell Biopolymer Nanoparticles Produced by Electrostatic Deposition of Beet Pectin onto Heat-Denatured β-Lactoglobulin Aggregates
Authors:R. Santipanichwong    M. Suphantharika    J. Weiss     D.J. McClements
Affiliation:Authors Santipanichwong and Suphantharika are with Dept. of Biotechnology, Faculty of Science, Mahidol Univ., Rama 6 Road, Bangkok 10400, Thailand. Authors Weiss and McClements are with Dept. of Food Science, Univ. of Massachusetts, Amherst, MA 01003, U.S.A. Direct inquiries to author McClements (E-mail: ).
Abstract:ABSTRACT:  The purpose of this study was to produce and characterize core-shell biopolymer particles based on electrostatic deposition of an anionic polysaccharide (beet pectin) onto amphoteric protein aggregates (heat-denatured β-lactoglobulin [β-lg]). Initially, the optimum conditions for forming stable protein particles were established by thermal treatment (80 °C for 15 min) of 0.5 wt%β-lg solutions at different pH values (3 to 7). After heating, stable submicron-sized ( d = 100 to 300 nm) protein aggregates could be formed in the pH range from 5.6 to 6. Core-shell biopolymer particles were formed by mixing a suspension of protein aggregates (formed by heating at pH 5.8) with a beet pectin solution at pH 7 and then adjusting the pH to values where the beet pectin is adsorbed (< pH 6). The impact of pH (3 to 7) and salt concentration (0 to 250 mM NaCl) on the properties of the core-shell biopolymer particles formed was then established. The biopolymer particles were stable to aggregation from pH 4 to 6, but aggregated at lower pH values because they had a relatively small ζ-potential. The biopolymer particles remained intact and stable to aggregation up to 250 mM NaCl at pH 4, indicating that they had good salt stability. The core-shell biopolymer particles prepared in this study may be useful for encapsulation and delivery of bioactive food components or as substitutes for lipid droplets.
Keywords:aggregation    β-lactoglobulin    biopolymer particles    core-shell    electrical charge    nanoparticles    pectin    stability
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