| 响应面法优化密蒙花总黄酮的超声提取工艺 |
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| 引用本文： | 郭雷,王淑军,刘玮炜. 响应面法优化密蒙花总黄酮的超声提取工艺[J]. 食品科学, 2012, 33(4): 13-17. DOI: 10.7506/spkx1002-6630-201204003 |
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| 作者姓名： | 郭雷 王淑军 刘玮炜 |
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| 作者单位： | 1.淮海工学院 江苏省海洋生物技术重点实验室 2.淮海工学院化学工程学院 |
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| 基金项目： | 江苏省高校自然科学基金项目(10KJB350001);江苏省海洋生物技术重点实验室基金项目(2010HS06) |
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| 摘 要： | 优化超声提取密蒙花总黄酮的工艺条件，在单因素试验基础上，考察乙醇体积分数、超声功率和提取温度3个因素对密蒙花总黄酮提取率的影响，并通过Box-Behnken试验设计和响应面分析法确定超声提取密蒙花总黄酮的最佳工艺条件。结果表明：最佳工艺条件为乙醇体积分数62%、超声功率410W、提取温度77℃、液料比50:1(mL/g)、提取时间35min，在此最佳提取条件下，总黄酮提取率为20.38%。
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| 关 键 词： | 密蒙花 总黄酮 超声提取 响应面分析法 |
Response Surface Optimization of Ultrasonic-Assisted Extraction of Total Flavonoids from Flos Buddlejae |
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| GUO Lei,WANG Shu-jun,LIU Wei-wei. Response Surface Optimization of Ultrasonic-Assisted Extraction of Total Flavonoids from Flos Buddlejae[J]. Food Science, 2012, 33(4): 13-17. DOI: 10.7506/spkx1002-6630-201204003 |
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| Authors: | GUO Lei WANG Shu-jun LIU Wei-wei |
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| Affiliation: | 1. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China；2. School of Chemistry and Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China |
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| Abstract: | This paper reports the optimization of conditions for the ultrasonic-assisted extraction of total flavonoids from Flos Buddlejae.One-factors-at-a-time experiments were carried out to investigate the effects of ethanol concentration,ultrasonic power and extraction temperature on extraction yield of total flavonoids.Further,the process parameters were optimized using a Box-Behnken experimental design coupled with response surface methodology.The optimal extraction conditions were determined as follows: ethanol concentration 62%,ultrasonic power 410 W,extraction temperature 77 ℃,liquid/material ratio(mL/g) 50:1,and extraction time 35 min.Under the optimal conditions,the extraction yield of total flavonoids from Flos Buddlejae was 20.38%. |
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| Keywords: | Flos Buddlejae total flavanoids ultrasonic-assisted extraction response surface methodology(RSM) |
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