| 碳离子束辐照选育截短侧耳素菌原生质体 |
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| 引用本文： | 都雯玥,陆锡宏,李雪虎,周翔,梁剑平,辛志君.碳离子束辐照选育截短侧耳素菌原生质体[J].辐射研究与辐射工艺学报,2016(1):39-44. |
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| 作者姓名： | 都雯玥 陆锡宏 李雪虎 周翔 梁剑平 辛志君 |
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| 作者单位： | 1. 中国科学院近代物理研究所兰州 730000;兰州理工大学生命科学与工程学院兰州 730000;2. 中国科学院近代物理研究所兰州 730000 |
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| 基金项目： | 国家高技术研究发展计划(863计划)(2011AA10A214)，甘肃省陇药产业发展专项(Y361010SJ0)项目资助
Supported by National High Technology Research and Development Program of China (863Program)(2011AA10A214)，Gansu Local Pharmaceutical Industry Development Fund(Y361010SJ0) |
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| 摘 要： | 使用12C6+离子束辐照Clitopilus pinsitus原生质体至不同吸收剂量,运用琼脂柱预筛和96孔板固体发酵选育截短侧耳素高产变异株。结果显示,最佳12C6+离子束吸收剂量为1.5 Gy,在该吸收剂量下正变异率为37.58%。选育出C.pin15I5E和C.pin15II6B两株正变异株,其产量较出发菌株分别提高16.17%和15.47%。表明重离子束辐照原生质体是行之有效的工业微生物诱变育种方法。
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| 关 键 词： | 碳离子束 辐照 Clitopilus pinsitus原生质体 高产变异株 截短侧耳素 |
Breeding pleuromutilin protoplasts strains by carbon ion beam irradiation |
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| Abstract: | Clitopilus pinsitusprotoplast was irradiated by12C6+ion beam at varied absorbed doses for mutagenesis, and high-yielding pleuromutilin mutant strains were screened using high-throughput method and solid fermentation. The results showed that the optimum12C6+ ion beam absorbed dose is 1.5 Gy, and under this condition the positive mutation rate is 37.58%. By agar column prescreening and 96-plate solid fermentation, the mutant strains, C.pin15Ⅰ5E and C.pin15Ⅱ6B, were selected. The yields of the strain increased by 16.17% and 15.47%, respectively, compared with that of the original strain. The research suggests that applying heavy ion irradiation to protoplast is a possible method to breed mutant strains. |
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| Keywords: | Carbon ion beam Irradiation Clitopilus pinsitus protoplast High-yielding mutant strain Pleuromutilin |
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