转基因大豆对硒的富集作用和形态分布研究

目的研究转基因大豆对硒的富集作用以及富集前后转基因大豆中硒的形态分布和形态转化。方法 采用电感耦合等离子体质谱分析转基因大豆富集前后硒的总量,采用蛋白酶提取,高效液相色谱-电感耦合等离子体质谱(high performance liquid chromatography-inductively coupled plasma-mass spectrometry,HPLC-ICP-MS)联用技术分析转基因大豆中的硒酸盐(selenate,Se VI)、亚硒酸盐(selenite,Se IV)、硒代蛋氨酸(selenomethionine,Se Met)和硒代胱氨酸(selenocystine,Se Cys)等几种硒化合物。盆栽种植试验研究转基因大豆对硒的富集作用。通过对照试验,考察大豆植株不同部位总硒含量变化和形态分布的转化情况。结果转基因大豆对硒有较好的富集作用,吸收的亚硒酸盐在大豆植株中部分转化成了硒代蛋氨酸和硒代胱氨酸。结论 了解了转基因大豆的形态分布和转化情况,可以更好地评估转基因大豆的食用安全风险并进行生物利用开发。

Study on selenium enrichment of genetically modified soybean and selenium species distribution analysis

Study on the selenium enrichment of genetically modified soybean, selenium compound species distribution and transformation before and after enrichment. The total selenium content in genetically modified soybean were detected by inductively coupled plasma-mass spectrometry, Extracted with protease, detected selenium species of selenite Se(IV)], selenateSe(VI)], selenomethionine(SeMet) and selenocystine (SeCys) in genetically modified soybean using high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). In order to research the change of total selenium content in different parts of the soybean plant and distribution of transformation of selenium species based on the contrast test, which were made by potted planting. The analysis results showed that the genetically modified soybean has better enrichment on selenium, selenite uptaked by soybean plant partly converted into selenomethionine and selenocystine. The analysis of distribution and transformation of selenium and its species will push forward on food safety risks and develop biological utilization of genetically modified soybean.