| 氧在重组毕氏酵母发酵过程中的摄取与传递 |
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| 引用本文： | 梁捷明,汪志锋,袁景淇. 氧在重组毕氏酵母发酵过程中的摄取与传递[J]. 化工学报, 2006, 57(10): 2397-2400 |
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| 作者姓名： | 梁捷明 汪志锋 袁景淇 |
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| 作者单位： | 上海交通大学自动化系，上海 200240;上海第二工业大学自动化系，上海 201209 |
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| 摘 要： | 引言 在生物发酵过程中,氧的供需是一个至关重要的问题[1-2].好气性微生物需要足够的溶解氧以维持自身的呼吸代谢.
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| 关 键 词： | 氧传递速率 摄氧率 毕氏酵母 重组人血清白蛋白 |
| 文章编号： | 0438-1157（2006）10-2397-04 |
| 收稿时间： | 2005-11-11 |
| 修稿时间： | 2005-11-112006-04-03 |
Oxygen uptake and transfer in recombinant Pichia pastoris cultivation |
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| LIANG Jieming,WANG Zhifeng,YUAN Jingqi. Oxygen uptake and transfer in recombinant Pichia pastoris cultivation[J]. Journal of Chemical Industry and Engineering(China), 2006, 57(10): 2397-2400 |
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| Authors: | LIANG Jieming WANG Zhifeng YUAN Jingqi |
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| Affiliation: | 1. Department of Automation, Shanghai Jiao Tong University, Shanghai 200240, China ; 2.Department of Automation, Shanghai Second Pol ytech University, Shanghai 201209, China |
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| Abstract: | Oxygen uptake and transfer rates were studied in recombinant Pichia pastoris cultivation carried out in a stirred tank bioreactor.The oxygen uptake rate was determined by the macrokinetic model proposed earlier.Based on pseudo-steady state mass transfer balance, an oxygen transfer model was established.Volumetric oxygen transfer coefficient k_La was estimated as a function of aeration rate and power input per unit volume.Oxygen uptake rate obtained from the macrokinetic model matched well with the oxygen transfer rate estimated by the mass balance model during the whole cultivation.The application of the oxygen transfer model for on-line biomass estimation was also presented. |
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| Keywords: | oxygen transfer rate oxygen uptake rate Pichia pastoris recombinant human serum albumin |
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