Superoxide radical production stimulates retroocular fibroblast proliferation in Graves'' ophthalmopathy |
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Authors: | HB Burch S Lahiri RS Bahn S Barnes |
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Affiliation: | Department of Genetics and Microbiology, Universitat Autónoma de Barcelona, Bellaterra, Spain. |
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Abstract: | The galE gene of Pasteurella multocida has been isolated by complementing galE-defective mutants of Salmonella typhimurium with a plasmid library of this organism. The complete nucleotide sequence of the P. multocida galE gene consists of 1017 nucleotides, encoding a predicted polypeptide of 339 amino acids. The deduced amino acid sequence displayed the highest identity (85%) to the GalE protein of Haemophilus influenzae. However, the gene organization surrounding the galE locus was different from that of H. influenzae. A galE-defective mutant of P. multocida was obtained by replacement of the active galE gene by a copy inactivated in vitro. The resulting galE mutant was highly attenuated as seen in a biological test carried out in a mouse model. |
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