首页 | 本学科首页   官方微博 | 高级检索  
     

当归胶囊中三种有效成分含量测定的方法学验证
引用本文:吴姣, 孙广庭, 陈迪安, 边禹. 当归胶囊中三种有效成分含量测定的方法学验证[J]. 食品工业科技, 2019, 40(23): 247-253. DOI: 10.13386/j.issn1002-0306.2019.23.040
作者姓名:吴姣  孙广庭  陈迪安  边禹
作者单位:1.美霖佳生物医学科技(苏州)有限公司, 江苏苏州 215000
摘    要:目的:建立当归胶囊中三种有效成分藁本内酯、阿魏酸、粗多糖的含量测定方法,并对检测方法进行方法学验证。方法:藁本内酯、阿魏酸采用高效液相色谱法,粗多糖采用紫外分光光度法,进行系统适应性、专属性、线性、检出限、准确度、精密度、重复性、稳定性等的方法学考察。结果:藁本内酯在20~60 μg/mL线性良好,r=0.9996,平均加样回收率为99.72%(RSD=2.99%),精密度、重复性、稳定性RSD均≤1%;阿魏酸在6~18 μg/mL线性良好,r=0.9990,平均加样回收率为96.11%(RSD=4.18%),精密度、重复性、稳定性RSD均≤2%;粗多糖在0.05~0.15 mg线性良好,r=0.9987,平均加样回收率为103.52%(RSD=4.79%),精密度、重复性、稳定性RSD均≤5%。结论:三种方法准确、可靠,可用于当归胶囊中三种有效成分藁本内酯、阿魏酸、粗多糖的含量测定。

关 键 词:当归  藁本内酯  阿魏酸  粗多糖  含量测定  方法学验证
收稿时间:2019-02-27

Method Validation for the Determination of Three Kinds of Active Ingredients in Angelica Capsules
WU Jiao, SUN Guang-ting, CHEN Di-an, BIAN Yu. Method Validation for the Determination of Three Kinds of Active Ingredients in Angelica Capsules[J]. Science and Technology of Food Industry, 2019, 40(23): 247-253. DOI: 10.13386/j.issn1002-0306.2019.23.040
Authors:WU Jiao  SUN Guang-ting  CHEN Di-an  BIAN Yu
Affiliation:1.Merrill Biomedical Technology(Suzhou) Co., Ltd., Suzhou 215000, China
Abstract:Objective:To establish methods for ligustilide,ferulic acid and polysaccharide content determination in angelica capsules and validate the methods,respectively. Methods:The HPLC analysis was performed for determination of ligustilide and ferulic acid,UV spectrophotometry was performed for determination of polysaccharide. Method validation included system adaptability,exclusivity,linearity,detection limit,accuracy,precision,repeatability and stability. Results:Good linearity of ligustilide was achieved in the range of 20~60 μg/mL(r=0.9996). The average recoveries was 99.72%(RSD=2.99%),RSD of precision,repeatability and stability were all less than 1%. Good linearity of ferulic acid was achieved in the range of 6~18 μg/mL(r=0.9990). The average recoveries was 96.11%(RSD=4.18%),RSD of precision,repeatability and stability were all less than 2%. Good linearity of polysaccharide was achieved in the range of 0.05~0.15 mg(r=0.9987). The average recoveries was 103.52%(RSD=4.79%),RSD of precision,repeatability and stability were all less than 5%. Conclusion:The three methods are accurate and reliable. It can be used to assay the content of ligustilide,ferulic acid and polysaccharide in angelica capsules,respectively.
Keywords:angelica  ligustilide  ferulic acid  polysaccharide  content determination  method validation
点击此处可从《食品工业科技》浏览原始摘要信息
点击此处可从《食品工业科技》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号