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Hypolipidemic and Anti-Obesity Effect of Anserine on Mice Orally Administered with High-Fat Diet via Regulating SREBP-1, NLRP3, and UCP-1 |
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| Authors: | Jing Luo Ming Chen Hongwu Ji Weifeng Su Wenkui Song Di Zhang Weiming Su Shucheng Liu |
| Affiliation: | 1. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088 P. R. China;2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088 P. R. China
Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, 524088 P. R. China;3. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088 P. R. China Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, 524088 P. R. China Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang, 524088 P. R. China Guangdong Provincial Engineering Technology Research Center of Marine Food, Zhanjiang, 524088 P. R. China Key Laboratory of Advanced Processing of Aquatic, Product of Guangdong Higher Education Institution, Zhanjiang, 524088 P. R. China Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034 P. R. China |
| Abstract: | To investigate the efficacy of anserine on antiobesity, C57BL/6 mice are orally administered with a high-fat diet (HFD) and different doses of anserine (60, 120, and 240 mg/kg/day) for 16 weeks. Body weight, lipid, and epididymal fat content in mice are measured, and their liver damage is observed. The results display that the body weight, epididymal fat content, and low-density lipoprotein cholesterol (LDL-C) content in anserine groups are decreased by 4.36–18.71%, 7.57–35.12%, and 24.32–44.40%, respectively. To further investigate the antiobesity mechanism of anserine, the expression of SREBP-1, NLRP3, NF-κB p65 (p65), and p-NF-κB p65 (p-p65) proteins in the liver and peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1-α) and UCP-1 proteins in brown adipose tissue (BAT) is analyzed by Western blot. Results show that anserine can significantly decrease the expression of the NLRP3, p65, p-p65, and the SREBP-1 proteins and increase the expression of the PGC1-α and UCP-1 proteins. This study demonstrates that anserine lowered blood lipids and prevented obesity; its antiobesity mechanism may be related to the activation of brown fat by inflammation. |
| Keywords: | anserine BAT lipid mice obesity |