当归多糖的分级制备及组成分析 THE PREPARATION, PURIFICATION AND COMPOSITION ANALYSIS OF ANGELICA SINENSIS POLYSACCHARIDES期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

当归多糖的分级制备及组成分析

引用本文：	孙元琳,申瑞玲,蔡宝玉,汤坚. 当归多糖的分级制备及组成分析[J]. 河南工业大学学报(自然科学版), 2005, 26(2): 40-44

作者姓名：	孙元琳申瑞玲蔡宝玉汤坚

作者单位：	1. 江南大学,食品学院,江苏,无锡,214036;山西运城学院,生命科学系,山西,运城,044000 2. 山西农业大学,动物科技学院,山西,太谷,030801 3. 江南大学,测试中心,江苏,无锡,214036 4. 江南大学,食品学院,江苏,无锡,214036

摘要：	当归多糖经离子交换柱层析得到3个多糖组分:W-ASP1由葡萄糖及少量阿拉伯糖和半乳糖组成,为中性多糖;W-ASP2主要由半乳糖、阿拉伯糖、葡萄糖及糖醛酸(35.38%)组成,为弱酸性多糖;W-ASP3主要含半乳糖、阿拉伯糖和鼠李糖,糖醛酸含量最高(58.27%),结合单糖组成分析确定是一种果胶多糖.酸水解结合HPAEC-PAD色谱测定当归多糖中的糖醛酸种类为半乳糖醛酸.通过苯酚-硫酸法绘制半乳糖醛酸的干扰标准曲线,消除GalA干扰得到多糖样品的中性糖含量,结合糖醛酸含量从而真实反映了样品的总糖含量.
关键词：	当归多糖分级糖醛酸中性糖总糖
文章编号：	1673-2383(2005)02-0040-04
修稿时间：	2004-12-09
THE PREPARATION, PURIFICATION AND COMPOSITION ANALYSIS OF ANGELICA SINENSIS POLYSACCHARIDES

SUN Yuan-lin,SHEN Rui-ling,CAI Bao-yu,TANG Jian. THE PREPARATION, PURIFICATION AND COMPOSITION ANALYSIS OF ANGELICA SINENSIS POLYSACCHARIDES[J]. Journal of Henan University of Technology Natural Science Edition, 2005, 26(2): 40-44

Authors:	SUN Yuan-lin SHEN Rui-ling CAI Bao-yu TANG Jian

Abstract:	Polysaccharides of Angelica sinensis were separated and analyzed by ion-exchange chromatography. The results showed that W-ASP1 were mainly composed of glucose and short amount of galactose and arabinose; W-ASP2 were composed of galactose, arabinose, glucose and uronic acid (35.38%); W-ASP3 were composed of galactose, arabinose, rhamnose, and high amount of uronic acid (58.27%). It was confirmed that W-ASP3 was a kind of pectic polysaccharide by monosaccharide composition analysis. Acid hydrolysis combined with HPAEC-PAD analysis showed that Angelica polysaccharides contained GalA. GalA interference measurement was made for determination of neutral polysaccharide, the total polysaccharide content was really reflected.

Keywords:	Angelica sinensis polysaccharides purification uronic acid neutral polysaccharide total sugars
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