桑叶酸性蛋白多糖(APFM)抗氧化作用的实验研究

目的:研究桑叶酸性蛋白多糖(APFM)的抗氧化作用。方法:在化学模拟实验中,测定APFM对羟自由基(·OH)和超氧阴离子自由基(O2·)的清除作用,并测定APFM对·OH诱导的小鼠脏器中MDA形成和积累的影响,以及对小鼠肝线粒体肿胀的抑制作用。在小鼠模型实验中,观察和分析APFM对四氧嘧啶所致糖尿病模型小鼠脏器中MDA含量和SOD活性等生理指标的影响。结果表明APFM可以有效清除化学模拟体系中形成的·OH和O2·,抑制小鼠脏器在该体系和糖尿病模型中MDA的形成和积累,明显减轻·OH诱导的小鼠肝线粒体的肿胀和模型小鼠脏器中SOD活性的降低,并都存在明显的量效关系。结论:可以认为APFM具有良好的体外和体内的抗氧化作用,是一种有效的自由基淬灭剂。

Antioxidant Activities of Acid Proteoglycan from Folium mori

Objective: To study the antioxidant activities of acid proteoglycan from Folium mori (APFM). Methods: In chemical mimicking experiments, the scavenging of hydroxyl free radical (·OH) and superoxide anion free radical (O2·) by APFM was determined. The effects of APFM on the production and accumulation of malondialdehyde (MDA) in mice organs induced by ·OH was examined, while also, the inhibition of APFM on the swelling of mice liver mitochondria induced by· HO was assayed. Through establishing mice model experiments, the effects of APFM on MDA content and superoxide dismutase (SOD) in the organs of diabetic mice induced by alloxan were observed and analyzed. Results: APFM can efficiently scavenge ·OH and O2· produced in chemical mimicking system, inhibit the production and accumulation of MDA both in mice organs induced by ·HO and in the organs of diabetic mice induced by alloxan, prominently lower the swelling of mice liver mitochondria induced by ·OH and increase the SOD activity in dose-dependent manner. Conclusion: APFM has strong antioxidant activity in vitro and in vivo, so it is an efficient free radical scavenger.