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| 酱香大曲中白地霉产蛋白酶固态发酵条件及其优化 | |
| 引用本文： | 张应莲,黄永光,邱树毅.酱香大曲中白地霉产蛋白酶固态发酵条件及其优化[J].中国酿造,2012,31(3):35-38. |
| 作者姓名： | 张应莲 黄永光 邱树毅 |
| 作者单位： | 1. 贵州大学 生命科学学院,贵州贵阳,550025 2. 贵州省轻工业科学研究所,贵州贵阳,550007 3. 贵州省发酵工程与生物制药重点实验室,贵州贵阳,550003 |
| 基金项目： | 国家科技支撑计划(2011BAC06B12号);贵州省工业攻关项目(黔科合GZ字(2011)3015)资助;黔科合重大专项((2010)6004号) |
| 摘 要： | 从酱香型酒大曲中分离得到一株白地霉MTBD,对其进行发酵实验,检测蛋白酶活力,测定其在不同原料比、不同培养时间、不同pH值、不同接种量、不同培养温度、不同料水比条件下产蛋白酶的情况,同时对其产酶条件进行优化,获得最佳产酶条件为：培养温度为45℃、pH值为3.5、料水比为1∶1、培养时间为6d。 |
| 关 键 词： | 酱香型白酒 白地霉 固态发酵 蛋白酶 正交试验 |
Solid-state fermentation condition and its optimization of protease production by Geotrichum candidum in Moutai-flavor Daqu |
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| ZHANG Yinglian , HUANG Yongguang , QIU Shuyi.Solid-state fermentation condition and its optimization of protease production by Geotrichum candidum in Moutai-flavor Daqu[J].China Brewing,2012,31(3):35-38. | |
| Authors: | ZHANG Yinglian HUANG Yongguang QIU Shuyi |
| Affiliation: | 1.College of Life Sciences,Guizhou University,Guiyang 550025,China;2.Guizhou Scientific Research Institute of Light Industry,Guiyang 550007,China;2.Guizhou Fermentation Engineering and Biotechnology Pharmaceutical Key Laboratories,Guiyang 550003,China) |
| Abstract: | A Geotrichum candidum Lnak MTBD was isolated from Daqu of Moutai-flavor liquor.The fermentation experiments were carried out for detection of protease activity under in the conditions of different raw materials,different fermentation time,different pH,different inoculum,different culture temperature,different ratios of material to water.And the enzyme production conditions were optimized,as well,which was as follows: culture temperature 45℃,pH 3.5,ratio of material to water 1∶1,fermentation time 6d. |
| Keywords: | Moutai-flavor liquor Geotrichum candidum solid-state fermentation protease orthogonal experiment |
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