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山楂叶中总黄酮的浸提工艺研究
引用本文:曹乐民,周志强,崔俊林,石明生.山楂叶中总黄酮的浸提工艺研究[J].中国酿造,2014(2):75-77.
作者姓名:曹乐民  周志强  崔俊林  石明生
作者单位:[1]河南农业职业学院,河南郑州451450 [2]重庆三峡职业学院,重庆404001
基金项目:河南省2012重点科技攻关项目(122102110164)
摘    要:以水提法研究山楂叶中总黄酮的含量,选取A、B、C这3种山楂叶,采用分光光度法测定山楂叶中总黄酮含量,以提取液中总黄酮含量为指标,通过正交试验优选其提取条件,并比较不同时期山楂叶中黄酮的总含量。样品B中黄酮含量最高,并通过正交试验设计分析得出最优浸提条件为90℃提取3h,pH8.5,料液比为1∶20。山楂叶中总黄酮物质提取率为10.375mg/g。

关 键 词:山楂叶  总黄酮  提取  分光光度法

Extraction technology of flavonoids from hawthorn leaves
CAO Lemin,ZHOU Zhiqiang,CUI Junlin,SHI Mingsheng.Extraction technology of flavonoids from hawthorn leaves[J].China Brewing,2014(2):75-77.
Authors:CAO Lemin  ZHOU Zhiqiang  CUI Junlin  SHI Mingsheng
Affiliation:1.Henan Vocational College of Agriculture, Zhengzhou 451450, China; 2.Chongqing Three Gorges Vocational College, Chongqing 404001, China;)
Abstract:To study total flavonoids content from hawthorn leaves by water extraction,the total flavonoids content was tested by spectrophotometer from 3 types of samples of A,B,C.Taking the total flavonoids content in extraction solution as an indicator for evaluation,the extraction conditions through orthogonal experiment was optimized,and the total flavonoids content among the hawthorn leaves in different periods was compared.Result showed that the total flavonoids content in sample B was the highest,and the optimal extraction condition was as follows:temperature 90℃,time 3h,pH 8.5,and solid to liquid ratio 1∶20.Under the condition,the total flavonoids content from hawthorn leaves was 10.375 mg/g.
Keywords:hawthorn leaves  total flavonoids  extraction  spectrophotometric method
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