| 基于纳米金颗粒放大的电化学传感器用于三聚氰胺的检测 |
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| 引用本文： | 王青,杨丽娟,羊小海,王柯敏,王辉. 基于纳米金颗粒放大的电化学传感器用于三聚氰胺的检测[J]. 化学传感器, 2013, 0(3): 17-21 |
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| 作者姓名： | 王青 杨丽娟 羊小海 王柯敏 王辉 |
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| 作者单位： | 湖南大学化学生物传感与计量学国家重点实验室,湖南大学化学化工学院,生物纳米与分子工程湖南省重点实验室,湖南长沙410082 |
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| 摘 要： | 三聚氰胺与胸腺嘧啶（T）之间能够通过三个氢键结合，以富T的DNA探针为识别元件，结合DNA修饰的纳米金颗粒放大技术，以电活性物质钌胺作为信号分子，发展了一种高灵敏检测三聚氰胺的电化学传感器，该传感器具有良好的特异性和灵敏度，检测下限低至0．5nmol／L。
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| 关 键 词： | 纳米金颗粒 电化学生物传感 三聚氰胺 |
An electrochemical biosensor for melamine detection based on gold nanoparticles signal amplification strategies |
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| Wang Qing,Yang L-juan,Yang Xiao-hai,Wang Ke-min,Wang Hui. An electrochemical biosensor for melamine detection based on gold nanoparticles signal amplification strategies[J]. Chemical Sensors, 2013, 0(3): 17-21 |
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| Authors: | Wang Qing Yang L-juan Yang Xiao-hai Wang Ke-min Wang Hui |
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| Affiliation: | (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China) |
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| Abstract: | Melamine could form triple H-bonds with thymine in aqueous medium. An electrochemical biosensor for melamine detection based on DNA modified gold nanoparticles amplification strategies was demonstrated. In this system, the T-rich DNA probes were immobilized on electrode surface as recognition element. The cationic electroactive complex [Ru（NH3）6]3＋ was bound to the DNA via electrostatic interactions as signal molecules. Since hundreds of DNA molecules were loaded on single gold nanopartiele, a good specificity and sensitivity was obtained in this method, the detection limit was ca. 0.5 nmol/L. |
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| Keywords: | Au nanoparticles electrochemical biosensor melamin |
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