| 生物素化普鲁兰多糖纳米颗粒制备与表征 |
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| 引用本文： | 史艳萍,李学敏,陈宝泉,黄玉萍,张其清. 生物素化普鲁兰多糖纳米颗粒制备与表征[J]. 功能材料, 2009, 40(1) |
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| 作者姓名： | 史艳萍 李学敏 陈宝泉 黄玉萍 张其清 |
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| 作者单位： | 史艳萍,陈宝泉,黄玉萍,SHI Yan-ping,CHEN Bao-quan,HUANG Yu-ping(天津理工大学,化学化工学院,天津,300191);李学敏,LI Xue-min(中国医学科学院生物医学工程研究所,天津,300192);张其清,ZHANG Qi-qing(中国医学科学院生物医学工程研究所,天津,300192;厦门大学医学院生物医学工程研究中心,福建,厦门,361005) |
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| 基金项目： | 国家重大科学研究计划，教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 合成生物素化普鲁兰多糖衍生物(BP),采用纳米沉淀法制备纳米颗粒(BPNs),考察制备条件对纳米颗粒性质影响,为进一步将其作为药物载体的研究提供基础.通过酯化反应将生物素羧基与普鲁兰多糖连接,生成的衍生物BP通过FI-IR和1H-NMR表征,取代度采用电感耦合等离子体光谱仪(ICP)确定;BPNs进行透射电镜、动态光散射仪和ζ电位仪表征与检测,颗粒表面生物素采用Quant*TagTM Biotin Kit生物素测定试剂盒测定.获得取代度21(BP1),46(BP2),81(BP3)3种衍生物,其中BP2和BP3能够制备纳米颗粒(LBPNs,HBPNs).纳米颗粒呈球形,表面光滑规整,平均粒径100～300nm,水中ζ电位在一17mV左右.制备过程中,粒径随BP浓度(10～50mg/ml)增加而增加((136.2±46.8)nm,(190.8±89.6)nm和(254.5±140.0)nm).调整水相组成为水:元水乙醇(v/v)1:1和1:2时粒径分别为(170.7±30.4)nm和(158.8±21.0)nm.HBPNs和LBPNs表面的生物素量为衍生物的(1.9±0.03)%和(2.0±0.04)%.生物素化普鲁兰多糖衍生物通过纳米沉淀法能制备出纳米颗粒,颗粒表面存在有生物素,颗粒性质受制备条件影响.
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| 关 键 词： | 生物素 普鲁兰多糖 纳米颗粒 纳米沉淀法 靶向 |
Preparation and characteristics of biotinylated pullulan nanoparticles |
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| SHI Yan-ping,LI Xue-min,CHEN Bao-quan,HUANG Yu-ping,ZHANG Qi-qing. Preparation and characteristics of biotinylated pullulan nanoparticles[J]. Journal of Functional Materials, 2009, 40(1) |
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| Authors: | SHI Yan-ping LI Xue-min CHEN Bao-quan HUANG Yu-ping ZHANG Qi-qing |
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| Affiliation: | 1.School of Chemistry and Chemical Engineering;Tianjin University of Technology;Tianjin 300191;China;2.Institute of Biomedical Engineering;Chinese Academy of Medical Science;Tianjin 300192;3.Research Center of Biomedical Engineering Medical School;Xiamen University;Xiamen 361005;China |
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| Abstract: | To synthesize biotinylated pullulan derivation(BP) and prepare nanoparticles by nanoprecipitation.The effects of different parameters on the nanoparticles formation were investigated for further ions for the BP as cancer target drug carriers.Methods Biotin was covalently attached to pullulan by esterification.FI-IR spectroscopy(Bio-Rad Merlin) and 1H-NMR(INOVA 500Hz) were used to confirm the synthesis of the BP.The degree of BP substitution(DS) was determined by ICP.The physicochemical properties of the nan... |
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| Keywords: | biotin Pullulan nanoparticles nanoprecipitation target |
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