Synthesis of phosphatidylglycerol from soybean lecithin with immobilized phospholipase D

Phosphatidylglycerol (PG) was synthesized from soybean lecithin with the help of phospholipase D (PLD) that was immobilized with calcium alginate gel-enveloped polyethyleneimine-glutarald ehyde. The immobilization conditions were optimized with response surface analysis: Ca-alginate 1.39%, polyethyleneimine 7.78%, glutaraldehyde 1.22%. The conversion of phosphatidylglycerol was 87%. The properties of the immobilized PLD were: optimal temperature 25–30°C, optimal pH 8.2, ratio of ether phase and water phase 1.5–2.5:1 (vol/vol), repeated 15 batches operating with stabilizing conversion percentage of phosphatidylglycerol.