基于高通量培养的胶球藻C-169生物量和油脂积累能力的快速评价

本研究利用CO_2培养箱内48孔板振荡光照培养专利设计的微藻高通量培养体系,结合细胞组学技术,系统评价了氮源和盐度梯度下胶球藻C-169细胞的生长速率、生物量浓度和油脂积累能力。结果表明,该培养体系的均一性和稳定性良好,可实现微藻的高通量及多处理培养。富氮培养基可显著提高胶球藻C-169的细胞生长速率和生物量浓度,缺氮培养基显著驱动了细胞积累中性脂。在含1/4氮的培养基中藻细胞的生物量浓度是4倍氮培养基中的25%,而中性脂含量(平均荧光强度表示)则高达后者的16倍以上。在0~15‰盐度范围内胶球藻C-169的生长状况良好且无显著差异(p0.05),15‰以上盐度藻细胞生长受到明显抑制。10‰盐度下中性脂含量(以平均荧光强度表示)显著高于所有其他组(p0.05),说明该盐度有利于驱动细胞积累中性脂。

Rapid Evaluation of Biomass and Lipid Accumulation Capacity in Coccomyxa subellipsoidea C-169 Cultured in a High-throughput System

Coccomyxa subellipsoidea C-169 cells were cultured using a high-throughput microalgal culture system with a patented design, 48-well microplate with shaking and illumination in a CO2 incubator. Combined with cytomics technology, the growth rate, biomass concentration, and lipid accumulation capacity in C. subellipsoidea C-169 cells under nitrogen and salinity gradients were systematically evaluated. The results showed that the culture system had excellent uniformity and stability, and could achieve high-throughput and multi-treatment microalgae culture. Cell growth and biomass concentration were significantly enhanced in nitrogen-rich medium; whereas neutral lipid accumulation was significantly enhanced in nitrogen-deficient medium. Biomass concentration in the medium containing 1/4 nitrogen concentration was 25% of that in medium containing four times as much nitrogen; however, neutral lipid content (shown as average fluorescence intensity) was more than 16 times higher. At salinity levels of 0~15%, C. subellipsoidea C-169 cells grew well, but rates were not significantly different (p>0.05). However, cell growth was significantly inhibited when salinity was higher than 15%. The neutral lipid content (shown as average fluorescence intensity) at a salinity of 10% was significantly higher than that of all other groups (p<0.05), indicating that this salinity level could facilitate accumulation of neutral lipids.