多孔分子印迹膜修饰的表面等离子体共振微囊藻毒素LR检测传感器

开发了一种多孔分子印迹膜修饰的表面等离子体共振(SPR)传感器,用于快速检测水中微囊藻毒素LR。研究了利用该传感器检测微囊藻毒素LR的方法。首先,通过原位聚合法在SPR传感芯片的裸金表面合成了微囊藻毒素LR的多孔分子印迹膜,制备出可以特异性捕获微囊藻毒素LR的SPR传感芯片。然后,利用Kretschmann棱镜耦合结构,构建了基于Kretschmann结构的波长调制型表面等离子体共振传感器。最后,通过检测不同浓度的微囊藻毒素LR溶液以及干扰物质微囊藻毒素RR溶液,研究了该传感器的测量范围、特异性等参数。结果表明,该传感器对于微囊藻毒素LR的检测灵敏度很高,可实现微囊藻毒素LR的定量检测,动态测量达2.1×10-9~1×10-6 mol/L。另外,传感器对于干扰物质微囊藻毒素RR无明显信号响应,表明传感器对于微囊藻毒素LR具有很好的特异性检测能力。

Surface plasmon resonance sensor coated with poriferous MIPs for microcystin-LR detection

A novel surface plasmon resonance (SPR) sensor coated with poriferous molecularly imprinted polymers (MIPs)was developed to detect the trace microcystin-LR in water. The method to detect the trace microcystin-LR in water was explored. Firstly, the in situ self-assembly method was used to synthesize a poriferous thin MIPs film on the gold-plated glass. The SPR chip to specifically capture the microcystin-LR was obtained. Then, based on the typical Kretschmann prism coupling structure, a new type of SPR sensor based on wavelength interrogation was constructed by utilizing the MIPs coated gold-plated glass as the sensing chip. The sensor was used to detect different concentration of microcystin-LR solutions and microcystin-RR solutions and to obtain its measuring ranges and specificity parameters. The result indicates that the sensor is sensitive to microcystin-LR and completes a quantitative determination with a dynamic measure range of 2.1×10^-9—1×10^-6 mol/L. Moreover, the sensor did not have obvious response to microcystin-RR, the analogues of microcystin-LR. It means that the sensor has special selectivity to recognize the microcystin-LR.