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Near real time in vivo fibre optic confocal microscopy: sub-cellular structure resolved |
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| Authors: | K B Sung C Liang† M Descour† T Collier M Follen‡ A Malpica‡ & R Richards-Kortum |
| Affiliation: | Department of Biomedical Engineering, University of Texas at Austin, Austin, TX 78712, U.S.A.;University of Arizona, Optical Sciences Center, 1630 East University Boulevard, Tucson, AZ 85721, U.S.A.;The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, U.S.A. |
| Abstract: | We have built a fibre optic confocal reflectance microscope capable of imaging biological tissue in near real time. The measured lateral resolution is 3 µm and axial resolution is 6 µm. Images of epithelial cells, excised tissue biopsies, and the human lip in vivo have been obtained at 15 frames s?1. Both cell morphology and tissue architecture can be appreciated from images obtained with this microscope. This device has the potential to enable reflected light confocal imaging of internal organs for in situ detection of pathology. |
| Keywords: | Confocal microscopy fibre optic in vivo laser reflectance scanning |