Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells |
| |
Authors: | Xue-Wen Yu Xin-Wen Zhang Xu Li |
| |
Affiliation: | 1. Center of Maternal and Child Health, First Affiliated Hospital of Medical School in Xi’an Jiaotong University, Xi’an, 710061, P. R. China; E-Mails: (X.-W.Y.); (X.-W.Z.);2. Center of Molecular Biological Medicine, First Affiliated Hospital of Medical School in Xi’an Jiaotong University, Xi’an, 710061, P. R. China |
| |
Abstract: | The tumor necrosis factor-alpha (TNF-α) cytokine receptor system modulates apoptosis in many cell types, so we have investigated the role of sTNFR1 in bacterial lipopolysaccharide (LPS)-induced cell death in cultured human decidual stromal cells, hypothesizing that sTNFR1 might play a central role in this action. In this work we characterized in vitro decidual stromal cell viability with LPS treatment and LPS and sTNFR1 co-treatment. We found that LPS treatment induced decidual stromal cell death in a dose-dependent manner and that sTNFR1 blocked the effect of the LPS treatment. There was a significant proliferation among cells co-incubated with LPS at 10 μg/mL and sTNFR1 at 0.1 μg/mL compared with LPS and sTNFR1 at 0.01, 0.05, 0.2 and 0.5 μg/mL (p < 0.01). This study demonstrated that LPS led to decidual stromal cell death in vitro but sTNFR1 down-regulates the cell death due to LPS under the same conditions. Taken together, these results suggested that sTNFR1 could participate in a protective mechanism against endotoxin. |
| |
Keywords: | Decidual stromal cell (DSC) soluble tumor necrosis factor receptor 1 (sTNFR1) membrane tumor necrosis factor receptor 1 (mTNFR1) pregnancy lipopolysaccharide (LPS) |
|
|