ISOLATION OF INTACT TYPE V COLLAGEN FROM FISH INTRAMUSCULAR CONNECTIVE TISSUE |
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| Authors: | KENJI SATO CHIHO OHASHI MITSUMI MURAKI HUKIKO ITSUDA YOSHIHIRO YOKOYAMA MASAO KANAMORI KOZO OHTSUKI MAKOTO KAWABATA |
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| Affiliation: | Department of Food Sciences and Nutritional Health Kyoto Prefectural University Shimogamo, Kyoto 606-8522 Japan;Interdisciplinary Research Institute for Biosciences Mukogawa Women's University Ikebiraki, Nishinomiya, Hyogo 663-8558 Japan |
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| Abstract: | A purification method for intact type V collagen from fish intramuscular connective tissue was developed. Collagens were extracted with 0.5 M acetic acid from fish intramuscular connective tissue. Intact type V collagen could not be isolated by conventional salt fractionation which has been used for isolation of pepsinized collagens. Isolation was achieved by ion-exchange chromatography in the presence of 5 M urea. This is the first report on isolation of intact type V collagen from muscle tissue. |
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