| 复合诱变选育表面活性素(surfactin)高产菌株 |
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| 引用本文： | 郭芳芳,李金良,陆兆新,别小妹,张充,吕凤霞.复合诱变选育表面活性素(surfactin)高产菌株[J].食品科学,2011,32(23):270-276. |
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| 作者姓名： | 郭芳芳 李金良 陆兆新 别小妹 张充 吕凤霞 |
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| 作者单位： | 南京农业大学食品科技学院 |
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| 基金项目： | “十二五”国家科技支撑计划项目(2011BAD23B05); 国家自然科学基金项目(30871753) |
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| 摘 要： | 为筛选抗菌脂肽表面活性素高产菌株，以枯草芽孢杆菌Bacillus subtilis fmbJ224为出发菌株，先后采用氯化锂(LiCl)、亚硝基胍(NTG)、甲基磺酸乙酯(EMS)和链霉素(streptomycin)进行复合诱变。结果表明：经初筛、复筛选育得到1株高产抗菌脂肽菌株LN2-3，其Surfactin产量达到239.2mg/L，比出发菌株Bacillus subtilis fmbJ224(5.25mg/L)提高了44.56倍，且该菌株具有良好的遗传稳定性。提示采用LiCl-NTG复合诱变能较大幅度的提高诱变率，可以获得抗菌脂肽高产菌株。
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| 关 键 词： | 芽孢杆菌 亚硝基胍(NTG) LiCl 复合诱变 表面活性素 |
Breeding of High-yield Surfactin-producing Strain by a Combined Mutagenesis Approach |
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| GUO Fang-fang,LI Jin-liang,LU Zhao-xin,BIE Xiao-mei,ZHANG Chong,LU..Feng-xia.Breeding of High-yield Surfactin-producing Strain by a Combined Mutagenesis Approach[J].Food Science,2011,32(23):270-276. |
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| Authors: | GUO Fang-fang LI Jin-liang LU Zhao-xin BIE Xiao-mei ZHANG Chong LUFeng-xia |
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| Affiliation: | (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China) |
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| Abstract: | In order to breed a high-yield surfactin-producing strain,Bacillus subtilis fmbJ224 was used as the start strain and mutagenized sequentially with lithium chloride(LiCl),nitrosoguanidine(NTG),ethyl methane sulphonate(EMS) and streptomycin.The preliminary and secondary screening results showed that strain LN2-3 had high ability to produce surfactin,with a yield of 239.2 mg/L,which revealed a 44.56-fold increase compared with the original strain.Meanwhile,the strain had good genetic stability.This study demon... |
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| Keywords: | Bacillus subtilis nitrosoguanidine(NTG) LiCl combined mutagenesis surfactin |
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