| 浒苔多糖的磷酸化修饰工艺 |
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| 引用本文： | 孙雪,潘道东,曾小群,曹锦轩.浒苔多糖的磷酸化修饰工艺[J].食品科学,2011(24). |
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| 作者姓名： | 孙雪 潘道东 曾小群 曹锦轩 |
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| 作者单位： | 宁波大学生命科学与生物工程学院;南京师范大学国家乳品加工技术研发分中心; |
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| 基金项目： | 浙江省自然科学基金重点项目(Z3110211) |
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| 摘 要： | 利用离子交换层析和凝胶层析对浒苔多糖进行分离纯化,并探讨其磷酸化修饰的最佳工艺条件。通过单因素试验确定反应温度、反应时间、反应pH值、磷酸化试剂质量浓度的取值范围,再用响应面设计法确定磷酸化修饰的最佳条件。结果表明:反应时间、磷酸化试剂质量浓度显著影响浒苔多糖磷酸化程度,当反应温度80℃、反应时间5h、反应pH9.0、磷酸化试剂质量浓度0.10g/mL时,磷酸化修饰的浒苔多糖磷酸根含量(以P计)达到14.40%。
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| 关 键 词： | 浒苔多糖 磷酸化 响应面法优化 |
Phosphorylation Modification of Polysaccharides from Entermorpha |
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| SUN Xue,PAN Dao-dong,,ZENG Xiao-qun,CAO Jin-xuan.Phosphorylation Modification of Polysaccharides from Entermorpha[J].Food Science,2011(24). |
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| Authors: | SUN Xue PAN Dao-dong ZENG Xiao-qun CAO Jin-xuan |
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| Affiliation: | SUN Xue1,PAN Dao-dong1,2,*,ZENG Xiao-qun1,CAO Jin-xuan1(1.College of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China,2.Branch of National Dairy Processing Technology Developing Center,Nanjing Normal University,Nanjing 210097,China) |
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| Abstract: | This study was undertaken to optimize the phosphorylation of Enteromorph polysaccharides prepared by hot water extraction,deproteinization,decolorization,and sequential chromatographic purification on DEA-Sepharose Fast Flow and Sephadex G-100 columns.Phosphate group content in phosphorylated products was investigated with respect to reaction conditions including temperature,reaction time,pH and phosphorylation agent dosage(sodium tripolyphosphate-sodium trimetaphosphate at a mass ratio of 6:1) by one-facto... |
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| Keywords: | polysaccharides from Entermorpha phosphorylation response surface methodology(RSM) |
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