| 从酸菜液中筛选产γ-氨基丁酸的菌株 |
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| 引用本文： | 梁金钟,田宇,王风青.从酸菜液中筛选产γ-氨基丁酸的菌株[J].食品科学,2011,32(23):244-249. |
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| 作者姓名： | 梁金钟 田宇 王风青 |
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| 作者单位： | 黑龙江省高校食品科学与工程重点实验室，哈尔滨商业大学食品工程学院 |
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| 基金项目： | 黑龙江省高校科技创新团队建设计划基金项目(2010td04) |
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| 摘 要： | 从腌制的酸菜液中，采用乳酸菌分离纯化法，经初筛、复筛得到一株产γ-氨基丁酸的菌株，编号为LpL-0212，对菌株进行形态学观察和生理生化实验及16S rDNA、atpA基因序列分析鉴定，鉴定该菌株为Enterococcus faecium。在含2%谷氨酸钠的TYG发酵培养基中静置培养24h，经薄层层析定性、高效液相色谱法测定，发酵液中的γ-氨基丁酸含量可达到102.37μmol/L。
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| 关 键 词： | γ-氨基丁酸 乳酸菌分离 鉴定 发酵液 |
Screening of a Gamma-Amino Butyric Acid-Producing Strain of Lactic Acid Bacteria from Pickled Vegetable |
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| LIANG Jin-zhong,TIAN Yu,WANG Feng-qing.Screening of a Gamma-Amino Butyric Acid-Producing Strain of Lactic Acid Bacteria from Pickled Vegetable[J].Food Science,2011,32(23):244-249. |
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| Authors: | LIANG Jin-zhong TIAN Yu WANG Feng-qing |
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| Affiliation: | (Key Laboratory of Food Science and Engineering, College of Food Engineering, Harbin University of Commerce,
Harbin 150076, China) |
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| Abstract: | A strain capable of producing γ-amino butyric acid (GABA), named as LpL-0212, was screened from pickled cabbage by a previously reported method for isolating and screening lactic acid bacteria. The strain was identified as Enterococcus faecium according to morphological observations, physiological and biochemical experiments, 16S rRNA sequence analysis, and atpA gene sequence analysis. Using quantitative thin layer chromatography and high-performance liquid chromatography, the content of GABA was determined to be 102.37 μmol/L in the fermentation broth obtained after 24 h culture of the strain in TYG medium containing 2% monosodium glutamate. |
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| Keywords: | γ-amino butyric acid lactic acid bacteria isolation identification fermentation broth |
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