| 肌醇六磷酸钙膜固定辣根过氧化酶制备过氧化氢生物传感器 |
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| 引用本文： | 接德丽,徐欢,董洁,文颖,杨海峰.肌醇六磷酸钙膜固定辣根过氧化酶制备过氧化氢生物传感器[J].应用化工,2011,40(8). |
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| 作者姓名： | 接德丽 徐欢 董洁 文颖 杨海峰 |
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| 作者单位： | 上海师范大学 生命与环境科学学院,上海,200234 |
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| 基金项目： | 国家自然科学面上基金项目(20975068) |
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| 摘 要： | 利用肌醇六磷酸钙(Ca-IP6)的磷酸酯键及Ca2+对HRP具有静电吸引和螯合作用,将其自组装于玻碳电极(GCE)表面,制备Nafion/HRP/Ca-IP6/GCE生物传感器。用紫外-可见吸收光谱、电化学阻抗光谱和循环伏安法对Nafion/HRP/Ca-IP6/GCE膜进行了表征。实验结果表明,吸附了HRP仍然能够保持原有的生物活性,实现了辣根过氧化物酶在电极表面的直接电子转移。该修饰电极对过氧化氢有很好的电催化活性,线性范围为2.67×10-7~1.067×10-6mol/L,最低检测限为1.3×10-7mol/L(信噪比S/N=3),米氏常数为0.259 mmol/L。该生物传感器拥有较低的检测限,较高的电催化效率和较好的稳定性及重现性。
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| 关 键 词： | 肌醇六磷酸钙 辣根过氧化物酶 生物传感器 直接电子转移 |
Hydrogen peroxide biosensor based on immobilization of horseradish peroxidase in calcium phytate matrix |
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| JIE De-li,XU Huan,DONG Jie,WEN Ying,YANG Hai-feng.Hydrogen peroxide biosensor based on immobilization of horseradish peroxidase in calcium phytate matrix[J].Applied chemical industry,2011,40(8). |
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| Authors: | JIE De-li XU Huan DONG Jie WEN Ying YANG Hai-feng |
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| Affiliation: | JIE De-li,XU Huan,DONG Jie,WEN Ying,YANG Hai-feng(Life and Environmental Science College,Shanghai Normal University,Shanghai 200234,China) |
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| Abstract: | A novel composite electrode of Nafion/HRP/Ca-IP6/GCE has been constructed for the electrochemical detection of H2O2.Nafion/HRP/Ca-IP6/GCE was characterized by UV-vis absorption spectroscopy,cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS).Experimental results showed that the absorbed HRP retained its bioactivity and realized direct electron transfer due to the improved biocompatibility of calcium phytate.Moreover,the biosensor displayed excellent bioelectrocatalytic ability toward the ... |
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| Keywords: | calcium phytate horseradish peroxidase biosensor direct electron transfer |
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