某低品位硫化铜矿混合菌浸出研究

利用混合菌对低品位硫化铜矿进行槽浸研究，并通过实时荧光定量PCR（RT-PCR）技术监测浸出过程中菌群的动态变化。研究结果表明，多种浸矿菌共同作用20 d，硫化铜矿中铜的生物浸出率达到85.66%，而酸浸仅为24.43%。在生物浸出的2～12 d，At.ferrooxidans是主要的浸矿菌，浸出液中Fe²⁺浓度快速下降、Eh上升，促进硫化矿的快速分解，Cu的浸出率达到79.50%；12 d以后，混合菌中的优势种逐渐被At.thiooxidans取代，浸出速度逐渐降低。此外，Mantel test结果表明，浸出液中Cu和Fe与微生物群落结构显著相关。

Bioleaching of a Low Grade Copper Sulphide Ore by Mixed Culture

The bioleaching of a kind of low grade sulphide ores was studied used a mixed culture of acidophilic microorganisms in a stirred tank reactor.RT-PCR（real-time fluorescence quantitative polymerase chain reaction) was used to identify the structure of the communities during bioleaching process.The results revealed that the bioleaching efficiency of Cu up to 85.66% in 20 d，and the acid-leaching without inocula only 24.43%.The bioleaching efficiency was up to 79.50% after 12 d bioleaching.At.ferrooxidans was the dominant with increase of Eh and decrease of Fe2+ during the leaching time from 2 to 12 d.After 12 d,At.thiooxidans gradually increased to replace At.ferrooxidans with the leaching rate decreased.Moreover，Mantel test indicated that the structure of the community were significantly correlated with Cu and Fe.